6、石蜡切片脱蜡至水：依次将切片放入二甲苯Ⅰ15min-二甲苯Ⅱ15m

6、石蜡切片脱蜡至水：依次将切片放入二甲苯Ⅰ15min-二甲苯Ⅱ15min-二甲苯III 15min-无水乙醇Ⅰ5min-无水乙醇Ⅱ5min-85%酒精5min-75%酒精5min-蒸馏水洗。抗原修复：组织切片置于盛满柠檬酸抗原修复缓冲液（PH6.0）的修复盒中于微波炉内进行抗原修复，中火8min至沸，停火8min保温再转中低火7min，此过程中应防止缓冲液过度蒸发，切勿干片。自然冷却后将玻片置于PBS（PH7.4）中在脱色摇床上晃动洗涤3次，每次5min。

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结果 (英语) 1: [复制]

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6, the paraffin sections were deparaffinized to water: The sections were placed in xylene Ⅰ15min- sequentially Ⅱ15min- xylene xylene III 15min- ethanol Ⅰ5min- Ⅱ5min-85% ethanol alcohol alcohol 5min- 5min-75% of distilled water. <br>Antigen Retrieval: tissue sections were filled with citric acid antigen retrieval cartridge retrieval buffer (PH6.0) in the microwave antigen retrieval in, fire 8min to boiling, cease-fire sub 8min low fire insulation 7min, this process buffer should prevent excessive evaporation, do not dry film. After natural cooling slides placed PBS (pH 7.4) in the bleaching shake shaker washed 3 times, each time 5min.

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结果 (英语) 2:[复制]

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6, paraffin slice dewax to water: in turn, the slices into xylene I.15min-xylene II.15min-xylene III 15min-aqueous ethanol I.5min-aqueous ethanol II.5min-85% alcohol 5min-75% alcohol 5min-distilled water wash.<br>Antigen repair: tissue slices placed in a repair box filled with citric acid antigen repair buffer (PH6.0) in the microwave oven for antigen repair, medium fire 8min to boiling, ceasefire 8min insulation and then turn low fire 7min, this process should prevent excessive evaporation of buffer, do not dry tablets. Naturally cool the slide in PBS (PH7.4) and shake and wash on the decoloring shaker 3 times, 5min each.

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结果 (英语) 3:[复制]

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6. Paraffin section dewaxing to water: successively put the section into xylene I 15min - xylene II 15min - xylene III 15min - anhydrous ethanol I 5min - anhydrous ethanol II 5min - 85% ethanol 5min - 75% ethanol 5min - distilled water washing.<br>Antigen repair: the tissue sections were placed in the repair box filled with citric acid antigen repair buffer (pH6.0) and repaired in the microwave oven. The medium fire lasted for 8 min to boiling, and the ceasefire lasted for 8 min and then turned to medium low fire for 7 min. in this process, excessive evaporation of buffer should be prevented and the chips should not be dried. After natural cooling, place the slide in PBS (pH7.4) and shake it on the bleaching shaker for 3 times, each time for 5min.

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