合成时，将固相载体填充至合成柱内，合成过程各项反应均在合成柱内进行，根

During the synthesis, the solid-phase carrier is filled into the synthesis column, and all reactions in the synthesis process are carried out in the synthesis column. According to the synthesis process, each base needs to undergo four-step reactions: deprotection→coupling→oxidation (or sulfuration)→ cap. The reagents used in the above four-step reaction include: dichloroacetic acid (DCA), 5-ethylthiotetrazolium (ETT) or 4.5 dicyanoimidazole (DCI), iodine, xanthane hydride (XH) Or phenylacetyl disulfide (PADS), 1-methylimidazole, acetic anhydride, 2.6-lutidine, these reagents are all dissolved in dichloromethane or acetonitrile or pyridine, and after each step of reaction is completed, 2-4 Two column volumes of acetonitrile to rinse residual reagents. Each four-step reaction is called a cycle, and it is repeated until the product sequence is synthesized, and finally decyanoethylated with diethylamine (DEA) or triethylamine (TEA) and then washed with acetonitrile for 2-4 times the column volume. Acetonitrile, often used as a solvent for various synthetic reagents, has a good dissolving effect on various reagents, and can wash away the residues of various synthetic reagents. There is less risk of reagent carryover or product carryover. Synthesizers have a separate risk assessment report on cleaning validation, see RAR-AMD-001 "OligoProcess Synthesizer Cleaning Validation Risk Assessment Report".

During synthesis, the solid phase carrier is filled into the synthesis column, and all reactions during the synthesis process are carried out within the column. According to the synthesis process, each base needs to undergo four steps of reaction: deprotection → coupling → oxidation (or vulcanization) → capping. The reagents used in the above four steps of reaction include dichloroacetic acid (DCA), 5-ethylthiotetrazolium (ETT) or 4.5 dicyanoimidazole (DCI), iodine, xanthan hydride (XH) or phenylacetyl disulfide (PADS), 1-methylimidazole, acetic anhydride, and 2.6-dimethylpyridine. These reagents are all dissolved in dichloromethane, acetonitrile, or pyridine. After each step of reaction, the residual reagents are washed with 2-4 times the column volume of acetonitrile. Each completion of four steps of reaction is called a cycle, which is repeated until the product sequence is synthesized. Finally, cyanoethyl is removed with diethylamine (DEA) or triethylamine (TEA), and the column volume is washed 2-4 times with acetonitrile again. Acetonitrile, often used as a solvent in the preparation of various synthetic reagents, has a good dissolution effect on various reagents and can rinse off residual synthetic reagents. The risk of reagent residue or product residue is relatively low. The synthesizer has a separate risk assessment report on cleaning validation, as shown in RAR-AMD-001 "OligoProcess Synthesizer Cleaning Validation Risk Assessment Report".

During synthesis, the solid carrier is filled into the synthesis column, and all the reactions in the synthesis process are carried out in the synthesis column. According to the synthesis process, each base needs to undergo four reactions: deprotection → coupling → oxidation (or vulcanization) → capping. The reagents used in the above four steps include: dichloroacetic acid (DCA), 5- ethylthiotetrazole (ETT) or 4.5 dicyanoimidazole (DCI), iodine, xanthane hydride (XH) or phenylacetyldisulfide (PADS), 1-methylimidazole, acetic anhydride and 2.6- dimethylpyridine, all of which are dissolved in dichloromethane, acetonitrile or pyridine, and each of them. Each four-step reaction is called a cycle, and this is repeated until the product sequence is synthesized. Finally, the column volume is washed with acetonitrile for 2-4 times after cyanoethylation with diethylamine (DEA) or triethylamine (TEA). Acetonitrile, often used as a solvent for the preparation of various synthetic reagents, has a good dissolution effect on various reagents, and can wash the residues of various synthetic reagents clean. The risk of reagent residue or product residue is small. The synthesizer has a separate risk assessment report on cleaning verification. See RAR-AMD-001 "Risk Assessment Report on Cleaning Verification of Oligoprocess Synthesizer".