In this study, a total of 17 natural populations (DH, LC, CT, YD, WP, RS, SX, QL, YP, SW, SC, NJ, ZN, JH, PY, DY, LA) were collected (Tables 1 and 1). Only 2 wild semi-maple loads were found in THE CT, ZN, YP population, so only 2 samples were taken, and only 3, 3, 4, 5, 7 and 8 wild semi-maple loads met the requirements were found in SW, YD, QL, DY, DH, and LA respectively. In the RS and LC populations, 10 and 19 samples were taken according to the sampling criteria, respectively, because the individuals were close to each other, and we wanted to avoid selecting potential cloned individuals. Sampling criteria: The geographic distance between the population is greater than 10 km, the spacing of the plants is greater than 10 m, and there are no pests and diseases. The leaves are immediately placed in a pre-prepared ice box, taken back to the lab, wiped the samples clean and wrapped in tin foil, then frozen with liquid nitrogen for 30 min, and then placed in an ultra-low temperature refrigerator to preserve and extract the DNA.
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