After the mixed reagent has no air bubbles, it is divided into 0.2ml eight-unit tubes, each tube is 15ul, placed at -80°C for 2 hours, and then freeze-dried.
After the mixed reagent has no bubbles, it is packed into 0.2ml eight-way tubes, each tube is 15ul, pre-cooled at -80℃ for 2 hours, and then freeze-dried.