If FAM has an amplification signal with Ct ≤ 36, and the amplification curve is a typical S-shape, the ORF1ab gene is (+); otherwise, it is (-) <br><br>if the Ct of FAM, JOE and ROX are all> 36, or no value ; Or Ct>32 of CY5, or no value, there is a problem with the sample or operation, and re-examination is required
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