弥弥切丸

みめいけい丸

3.1. ZTO had antifungal activity against P. capsici<br><br>Since ZTO had inhibitory effect on vegetative growth of P. capsici, it was probable that ZTO could impact the process of reproductive growth. First, the effect of ZTO on sporangium formation of P. capsici was examined. Results showed that the formation of sporangium sig-nificantly reduced by the treatment of ZTO with a dose-dependent manner, and no sporangium was observed after treated with 10 μg/mL and 20 μg/mL of ZTO (Fig. 1B). Second, we tested the effect of ZTO on zoospore production. As shown in Fig. 1C, the number of zoospores significantly decreased in a dose-dependent manner. Similarly, 10 μg/ mL and 20 μg/mL of ZTO completely inhibited the release of zoospores (Fig. 1C).<br>To further investigate the effect of ZTO on zoospore germination, the zoospores were prepared and treated with ZTO, and the germina-tion rates of zoospores were monitored in a time course experiment. Results showed that it took 10, 12, 24 and 24 h to achieve 100% ger-mination rates at 2.5, 5, 10 and 20 μg/mL of ZTO, respectively, when the control group only took 6h to achieve 100% germination rate (Fig. 1D). Overall, our results suggested that ZTO could suppress not only mycelial growth but also reproductive growth of P. capsici. ...

弥切丸<br>