从225对SRAP引物中筛选出扩增条带清晰、多态性高的引物10对。10

Of 225 pairs of SRAP primers were successfully amplified bands were clear, high polymorphism Primer 10 pairs. Polymorphism clear strip 10 SRAP primer pairs produced with polymorphism and PIC percentage shown in Table 3. 154 parts of 10 pairs of SRAP marker Pterospermum germplasm was amplified to obtain tape strip 179 has a polymorphism. Number of strips per primer combination range is 17 (Me1-Em28 and Me8-Em4) to 19 (Me15-Em6), per combination of primers amplified from 17.9 tape. PIC discloses ability to distinguish different primer combinations. The average was 0.44 PIC all tags. PIC values ​​Me13-Em1 at most 0.499, followed Me11-Em14 PIC value of 0.486, the lowest value PIC primer composition and Me8-Em4 Me15-Em6 was 0.454.

From 225 pairs of SRAP primers, 10 pairs of primers with clear and polymorphic high profile were screened out. 10 Clear polymorphism bands, polymorphism percentages, and PIC produced for SRAP primers are shown in Table 3. Ten SRAP markers were amplified on 154 semi-maple materials, and 179 strips were polymorphic. The number of bands per pair of primers is 17 (Me1-Em28 and Me8-Em4) to 19 (Me15-Em6), with an average of 17.9 bands amplified per pair of primers combined. PIC reveals the ability to distinguish between different primer combinations. The average PIC value for all tags is 0.44. Me13-Em1 has a highest PIC value of 0.499, followed by Me11-Em14 with a PIC value of 0.486, and a pic value of 0.454 for the primer combination Me8-Em4 and Me15-Em6.

Ten pairs of primers with clear bands and high polymorphism were screened out from 225 pairs of SRAP primers. The clear polymorphic bands, percentage of polymorphisms and PIC produced by 10 pairs of SRAP primers are shown in Table 3. Ten pairs of SRAP markers were amplified from 154 germplasm materials of schizophrenia superba and 179 bands were polymorphic. The number of bands per pair of primer combinations ranged from 17 (me1-em28 and me8-em4) to 19 (me15-em6), with an average of 17.9 bands per pair of primer combinations. Pic revealed the distinguishing ability of different primer combinations. The average pic value of all markers was 0.44. The highest pic value of me13-em1 was 0.499, the second was 0.486 of me11-em14, and the lowest pic value of primer combination me8-em4 and me15-em6 was 0.454.<br>