The initial acidification of pyrite is a natural oxidation process mediated by O2, and the reaction rate is extremely slow. The oxidation of FeS2 is mainly completed by the oxidation of Fe3 +, and the role of acidophilic microorganisms is that it can accelerate the oxidation of Fe2 + to Fe3 + (Johnson and Hallberg, 2003). Under the action of microorganisms, this rate is 5 orders of magnitude faster than the initial acidification process (singer and stump, 1970).<br>
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