From 112 plant endophytic fungi, 12 oil-producing strains with an oil-producing rate of more than 20% were obtained, and the neutral lipid content was determined to be 38.7%-79.6%. Three strains of endophytic fungi Fusa5rium sp., Dothideomycetes sp., Aspergillus sp. were screened from them, and the best cultivation plan was obtained by optimizing the culture conditions. The oil yield of Dothideomycetes sp. reached 51.66%, and that of Fusa5rium sp. The oil rate reached 67.58%, and the oil production rate of Aspergillus sp. reached 61.19%. And calculate the predicted biodiesel properties based on the measured fatty acid profile. This study shows that these three novel endophytic fungi can be used as potential biodiesel raw materials.
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