第二，大量的筛选验证工作被开展，其中，可以在蔗糖平板生长的、带有同源臂

Second, a large amount of screening and verification work has been carried out. Among them, some suspected positive strains that can grow on sucrose plates with homologous arm genes without relA genes or plasmid sequences are screened. <br><br>However, these positive strains reappear the relA gene or return to the wild type after continuing isolation or incubation, and further optimization and testing are still needed in the follow-up.

Second, a large number of screening and verification work has been carried out, in which some suspected positive strains with homologous arm genes grown on the sucrose plate without the relA gene or plasmid sequence can be screened.<br><br>However, these positive strains reappear or return to wild form after continued isolation or incubation, and further optimization and testing are required.

Secondly, a large number of screening and validation work has been carried out, in which some suspected positive strains with homologous arm gene but no rela gene or plasmid sequence can be screened.<br>However, after further isolation or incubation, the rela gene of these positive strains reappeared or returned to the wild type, and further optimization and experiments are still needed.<br>