根据生化试验、PCR确认试验的结果，25g 样品中是否检出或未检出某类

According to the results of the biochemical test and PCR confirmation test, whether or not a certain type of diarrhea-causing Escherichia coli was detected in the 25g sample was reported. <br>If a serological test is performed, according to the results of the serological test, a certain type of diarrhea-causing Escherichia coli serotype detected in the 25g (or 25mL) sample is reported. <br>Appendix A <br>A. 1 Nutritional Broth A.1.1 Ingredients <br>Peptone <br>Beef Paste <br>Sodium Chloride <br>Distilled Water <br>A.1.2 Preparation Method <br>The above ingredients are mixed and heated to dissolve, cooled to about 25 ℃ to correct the pH to 7.4 ± 0.2, and packed into appropriate containers. <br>Sterilize at 121 ° C for 15 minutes. <br>A.2 Intestinal Bacterial Enrichment Broth A .2.1 Ingredients <br>Glucose <br>Bile Salt <br>Disodium Hydrogen Phosphate <br>Potassium Dihydrogen Phosphorus <br>Green <br>A. 2.2 Preparation Method <br>Mix and dissolve the above ingredients by heating, cooling to about 25 ° C to correct pH to 7.2 0.2, aliquot 30mL per bottle. Sterilize at 115 ℃ for 20 min. <br>A3 MacConkey Agar (MAC) <br>A.3.1 Ingredients <br>Lactose <br>No. 3 Bile Salt <br>Neutral Red <br>Crystal Violet <br>Agar <br>The above ingredients are mixed and heated to dissolve, and the pH is corrected to 7.2 ± 0.2. <br>Autoclave at 121 ℃ for 15min.

According to the results of biochemical tests and PCR confirmation tests, whether or not some type of ejaculation easis was detected in the 25g sample.<br>If a serological test is performed, a certain type of celiac ethyl ehesis serotype detected in a 25g (or 25mL) sample is reported based on the results of the serological test.<br>Appendix A<br>A. 1 Nutritional broth A.1.1 ingredients<br>Peptone<br>Beef paste<br>Sodium chloride<br>Distilled water<br>A.1.2 Law<br>Mix the above ingredients to dissolve, cool edgy to about 25 degrees C correction pH to 7.4 x 0.2, divided into appropriate containers.<br>Sterilization 1 5min at 121 degrees C.<br>A.2 Intestinal bacteria-enhanced broth A .2.1 cents<br>Glucose<br>Cow bile salt<br>Sodium phosphate<br>Potassium dihydrophosphate<br>Huang Green<br>A .2.2<br>The above ingredients are mixed and heated to dissolve, cooled to about 25 degrees C correction pH to 7.2 to 0.2, divided into 30 mL per bottle. Sterilization 20 min at 115 degrees C.<br>A3 McConkey Agar (MAC)<br>A.3.1 Ingredients<br>Lactose<br>No. 3 bile salt<br>Neutral Red<br>Crystal purple<br>Agar<br>The above ingredients are mixed and heated to dissolve, correcting pH to 7.2 to 0.2.<br>15min autoclave at 121 degrees C.

According to the results of biochemical test and PCR confirmation test, whether a certain type of diarrhea causing Escherichia coli was detected or not in 25g sample was reported.<br>If a serological test is carried out, according to the results of the serological test, a certain type of diarrhea causing Escherichia coli serotype detected in 25g (or 25ml) sample is reported.<br>Appendix A<br>A. 1. Ingredients of nutritious broth a.1.1<br>Peptone<br>Beef paste<br>sodium chloride<br>distilled water<br>A. 1.2 system<br>Mix and heat the above ingredients to dissolve them, cool them to about 25 ℃ and correct the pH to 7.4 ± 0.2, and separate them into appropriate containers.<br>Sterilization at 121 ℃ for 15 min.<br>A. 2. Ingredient A.2.1<br>glucose<br>Bile salt<br>Disodium hydrogen phosphate<br>Potassium dihydrogen phosphate<br>Brilliant green<br>A.2.2 system<br>Mix and heat the above ingredients to dissolve them, cool them to about 25 ℃ and correct the pH value to 7.2 ± 0.2, and separately pack each bottle of 30ml. Sterilize at 115 ℃ for 20 minutes.<br>A3 MacConkey agar (MAC)<br>A. 3.1 composition<br>lactose<br>Bile salt No.3<br>neutral red<br>crystal violet<br>agar<br>Mix and heat the above ingredients to dissolve, and adjust the pH to 7.2 ± 0.2.<br>Autoclave at 121 ℃ for 15 min.<br>