We designed the mutation site to be detected at the 3' end of the FIP primer, and made artificial changes at the adjacent bases at the 3' end of the FIP primer so that it could not be complementary to the template.
We designed the mutation site to be detected at the 3 'end of FIP primer, and artificially changed the adjacent base at the 3' end of FIP primer, so that it can not be complementary to the template.
We designed the mutation site to be detected at the 3' end of FIP primer, and artificially changed the base adjacent to the 3' end of FIP primer, so that it could not complement the template.