将适量的玻璃珠添加至含0.05%（ml/ml）聚山梨酯80的pH7.0

Add an appropriate amount of glass beads to the pH 7.0 sterile sodium chloride-peptone buffer containing 0.05% (ml/ml) polysorbate 80 or 0.9% sterile containing 0.05% (ml/ml) polysorbate 80 Sodium chloride solution, after shaking the bacteria liquid, gently press the inclined surface with sterile gauze, suck the bacteria liquid on the gauze to make a concentrated spore suspension, check the purity according to item 7.5.3, and check the qualified spore suspension Concentrated spore suspension required for the experiment. <br>black

Add the appropriate amount of glass beads to pH7.0 0 sterile sodium chloride胨 buffer containing 0.05% (ml/ml) polysorbester 80 or 0.9 with 0.05% (ml/ml) polysorbester 80 % sterile sodium chloride solution, shake the bacterium solution, with sterile gauze gently press the slope, absorb the bacteria on the gauze, to make a thick spore suspension, according to 7.5.3 items for purity check, purity check qualified spore suspension for the experiment required thick spore suspension.<br>Black.

Add appropriate amount of glass beads to pH 7.0 sterile Sodium Chloride Peptone buffer containing 0.05% (ml / ml) polysorbate 80 or 0.9% sterile sodium chloride solution containing 0.05% (ml / ml) polysorbate 80. After shaking the bacterial solution, gently press the inclined plane with sterile gauze, absorb the bacterial liquid on the gauze, and prepare concentrated spore suspension. The purity test is conducted according to 7.5.3. The spore suspension with qualified purity inspection is solid The required concentrated spore suspension was tested.<br>black<br>