PCR reaction procedures for this test: 7 c prevariction 7 min; 7 cycles (94 degrees C denaturation 45 s, 35 degrees C annealing 1 min; 72 degrees C extension 1 min); 28 cycles (94 degrees C denatured 45 s, 35 degrees C annealing 1 min; 52 degrees C extension 1 min) 72 degrees C extension 10 min.
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