弃去原培养液。分别加入空白对照液、阴性对照、阳性对照液、供试品液各3皿

Discard the original culture medium. <br>Add 3 dishes each of blank control solution, negative control, positive control solution, and test product solution, 2 mL per dish (if the extraction medium does not contain serum, add 1.8 mL extraction medium and 0.2 mL calf serum to each dish). <br>Place the CO 2 incubator at 37°C and incubate for 48 hours. <br>After incubation, observe the changes of cell morphology, vacuole formation, shedding, cell lysis and membrane integrity under a microscope, and judge the results according to the following criteria: <br>cytotoxicity reaction classification/scoring

Discard the original culture.<br>Add blank control fluid, negative control, positive control fluid, test liquid each 3 dishes, each dish 2mL (e.g. leaching medium without serum, each dish added 1.8mL leaching medium and 0.2mL calf serum).<br>CO 2 culture box 37 degrees C culture 48h.<br>After the culture is over, the changes in cell morphology, empty bubble formation, shedding, cell dissolution and membrane integrity are observed under the microscope靣, and the results are judged according to the following criteria:<br>Cytotoxic reaction grading/scoring

Discard the original culture medium.<br>Add 3 plates of blank control solution, negative control solution, positive control solution and test solution respectively, 2ml each plate (if the extraction medium does not contain serum, add 1.8ml extraction medium and 0.2ml calf serum into each plate).<br>The cells were cultured in CO2 incubator at 37 ℃ for 48 h.<br>After culture, the changes of cell morphology, vacuole formation, abscission, cell dissolution and membrane integrity were observed under microscope<br>Cytotoxicity grading / scoring<br>