3. The basic principles and classif

3. The basic principles and classification of<br>chemiluminescence immunoassay analyzers Chemiluminescence immunoassay products have been commercialized and industrialized internationally, and can basically be divided into chemiluminescence immunoassay and electrochemiluminescence immunoassay. Chemiluminescence is a chemical reaction (usually an oxidation reaction) that produces substances whose electronic energy levels are in an excited state. The The latter releases energy through electronic transitions to generate photons, leading to luminescence. The analytical method established based on the relationship between the chemiluminescence intensity and the content of the analyte is called chemiluminescence analysis. It has the characteristics of high sensitivity, wide linear range, simple instrument and convenient operation. It has been widely used in environmental, clinical,food and industrial analysis. According to the type of reaction, chemiluminescence can be divided into enzymatic chemiluminescence and non-chemiluminescence. Among them, enzymatic chemiluminescence mainly includes horseradish peroxidase (HRP) system and alkaline-encoding oxidase (AP) system. Non-enzymatic chemiluminescence mainly includes the acridine system. According to the duration of light emission, it can be divided into flash and glow type. Among them, the flash emission time is short, in seconds, such as the acridine system. Generally, the in-situ sampling Cinmsituinjector and the heart-to-heart integration method are used for measurement, that is, an injector is installed at the detector to ensure that the two processes of adding luminescent agent and detecting are carried out simultaneously. At the same time,the area of ​​​​the entire luminescence signal peak is the luminescence intensity: the glow-type luminescence time is several minutes to several tens of minutes, such as horseradish peroxidase luminol system and alkaline phosphatase AMPPD system. Signal detection does not require in-situ sampling. Generally, the rate method is used to measure the luminous intensity per unit time (flat area) at any point in an area where the luminous signal is relatively stable.the rate method is used to measure the luminous intensity per unit time (flat area) at any point in an area where the luminous signal is relatively stable.the rate method is used to measure the luminous intensity per unit time (flat area) at any point in an area where the luminous signal is relatively stable.

3. The basic principles and classification of <br>chemiluminescence immunoassay analyzers Chemiluminescence immunoassay products have been commercialized and industrialized internationally, and can basically be divided into chemiluminescence immunoassay and electrochemiluminescence immunoassay. Chemiluminescence is a chemical reaction (usually an oxidation reaction) that produces substances whose electronic energy levels are in an excited state. The latter releases energy through electronic transitions to generate photons, leading to luminescence. The analytical method established based on the relationship between the chemiluminescence intensity and the content of the analyte is called chemiluminescence analysis. It has the characteristics of high sensitivity, wide linear range, simple instrument and convenient operation. It has been widely used in environmental, clinical, food and industrial analysis. According to the type of reaction, chemiluminescence can be divided into enzymatic chemiluminescence and non-chemiluminescence. Among them, enzymatic chemiluminescence mainly includes horseradish peroxidase (HRP) system and alkaline-encoding oxidase (AP) system. Non-enzymatic chemiluminescence mainly includes the acridine system. According to the duration of light emission, it can be divided into flash and glow type. Among them, the flash emission time is short, in seconds, such as the acridine system. Generally, the in-situ sampling Cinmsituinjector and the heart-to-heart integration method are used for measurement, that is, an injector is installed at the detector to ensure that the two processes of adding luminescent agent and detecting are carried out simultaneously. At the same time, the area of ​​the entire luminescence signal peak is the luminescence intensity: the glow-type luminescence time is several minutes to several tens of minutes, such as horseradish peroxidase luminol system and alkaline phosphatase AMPPD system. Signal detection does not require in-situ sampling. Generally, the rate method is used to measure the luminous intensity per unit time (flat area) at any point in an area where the luminous signal is relatively stable.

三。基本原理和分类<br>化学发光免疫分析仪器化学发光免疫分析产品在国际上已实现商品化和产业化，基本可分为化学发光免疫分析和电化学发光免疫分析。化学发光是一种化学反应（通常是氧化反应），产生电子能级处于激发态的物质。后者通过电子跃迁释放能量，产生光子，导致发光。根据化学发光强度与被测物含量的关系建立的分析方法称为化学发光分析法。它具有灵敏度高、线性范围宽、仪器简单、操作方便等特点。它已广泛应用于环境、临床、食品和工业分析。根据反应类型的不同，化学发光可分为酶化学发光和非化学发光。其中酶化学发光主要包括辣根过氧化物酶（HRP）体系和碱性编码氧化酶（AP）体系。非酶化学发光主要包括吖啶体系。根据发光的持续时间，可分为闪光型和辉光型。其中，闪光发射时间短，以秒计，如吖啶体系。一般采用原位进样注射器和心-心积分法进行测量，即在检测器上安装一个注射器，保证加入发光剂和检测两个过程同时进行。同时，整个发光信号峰的面积就是发光强度：发光类型的发光时间为几分钟到几十分钟，如辣根过氧化物酶-鲁米诺体系和碱性磷酸酶-AMPPD体系。信号检测不需要现场采样。通常，速率法用于在发光信号相对稳定的区域中的任何点测量单位时间（平坦区域）的发光强度。<br>