摘 要 ：布鲁氏菌外膜蛋白OMP25是布鲁氏菌中一种重要的免疫原性和保

Abstract: <br>Brucella outer membrane protein OMP25 is an important Brucella immunogenicity and protective antigen, it can prevent the production of pro-inflammatory cytokines, promote cell survival and immune evasion. OMP25 are conserved protein found in all the major Brucella, it is one of the candidate subunit vaccine have developed promising. In this study Brucella OMP25 genes from patients with clinical training were identified and cloned protein expression, successfully acquired a complete OMP25 gene, encoding gene is 657bp, sequencing analysis showed that Brucella coding and published NCBI Repository OMP25 gene homology of 100%; the expressed product by SDS-PAGE and Western blot analysis proved consistent with the theoretical value of about 24.1 kDa; Ni-NTA column purification and anti purified by, the OMP25 protein in E.Coli expression yield of up to 200mg / L. Through advanced software to predict the structure of OMP25 protein epitope prediction analysis of OMP25 protein. This research laid the foundation for the preparation of OMP25 subunit vaccine.

Excerpt:<br>Brucellosis exomembrane protein OMP25 is an important immunogenandamy and protective antigen in brucellosis, which can prevent the production of pro-inflammatory cytokines, promote the survival of the bacteria and immune escape. The OMP25 protein is conservatively present in all major brucellosis and is one of the promising subunit vaccines. In this study, the olyscocci OMP25 gene from clinical patient identification culture was cloned and protein expression, and the OMP25 complete gene was successfully obtained, coding the base because 657bp, Sequencing analysis showed that the OMP25 gene sequence of brucellosis encoded in the NCBI data bank was 100% homogenous, the expression product signed by SDS-PAGE and Western blot analysis, which proved to be consistent with theoretical calculations, was about 24.1 kDaa, and the Expression yield of OMP25 protein in E.Coli was up to 200mg/L. The advanced structure of OMP25 protein is predicted by software, and the antigen epitope of OMP25 protein is predicted and analyzed. The research results lay the foundation for the preparation of OMP25 subunit vaccine.

Abstract:<br>Omp25 is an important immunogenic and protective antigen in Brucella. It can prevent the production of proinflammatory cytokines, promote the survival and immune escape of Brucella. Omp25 protein is conserved in all major Brucella, and it is one of the promising candidate subunit vaccines. In this study, the omp25 gene of Brucella from clinical patients was cloned and expressed, and the complete omp25 gene was successfully obtained. The coding gene was 657 BP. Sequencing analysis showed that the sequence homology of omp25 gene with that of Brucella published in NCBI information database was 100%. SDS-PAGE and Western blot analysis showed that the expression product was consistent with the theoretical calculation value, The expression of omp25 protein in E.coli was up to 200 mg / L after purification and reverse purification on Ni NTA column. The advanced structure and epitope of omp25 were predicted by software. The research results lay a foundation for the preparation of omp25 subunit vaccine.<br>