取适量JC-1（200×），按照每50μL JC-1（200 ×）加入

Take an appropriate amount of JC-1 (200×), and dilute JC-1 at the ratio of adding 8mL ultrapure water for every 50μL of JC-1 (200×). Shake vigorously to dissolve and mix JC-1 thoroughly. Then add 2mL JC-1 staining buffer (5 ×), and mix well to become the JC-1 staining working solution.

Take the appropriate amount of JC-1 (200×) and dilute JC-1 in proportion to 8mL ultra-pure water per 50 μL JC-1 (200 ×). Severe shock fully dissolves and mixes JC-1. Then add 2mL JC-1 dye buffer (5 ×), and after mixing, JC-1 dyeing fluid.

Take an appropriate amount of JC-1 (200 ×) and dilute JC-1 with 8ml ultra pure water per 50 μ l JC-1 (200 ×). The JC-1 was thoroughly dissolved and well mixed by violent shaking. Then add 2ml JC-1 dyeing buffer (5 ×), and mix it to JC-1 dyeing working solution.