但是，形态上已经形成的纳米粒并不稳定，还需要通过戊二醛等交联而硬化。但

但是，形态上已经形成的纳米粒并不稳定，还需要通过戊二醛等交联而硬化。但是，不利影响，如生物活性大分子失活和有害溶剂的残留会被造成。总之，丙酮、氯仿和二氯甲烷等中高毒性的有机溶剂需被以上的方法要求在制备过程中加入，一定污染会据此造成。绿色化学是制药行业的强大盟友。从绿色化学的角度来看，两个主要步骤须在制备纳米颗粒过程中完成：选择用于合成的溶剂介质以及选择用于稳定纳米颗粒的无毒材料。迄今为止报道的大多数制备方法严重依赖有机溶剂。与此相比，开发环境友好和可持续的方法需被提出以制备纳米粒子在绿色合成策略中。在本研究中，一种新的绿色的制备白蛋白微粒的方法（高压均质-凝固法）被提出。在水体系中，白蛋白纳米颗粒被形成，方法是利用凝固剂的作用，使白蛋白分子凝固，同时利用高压均质的空化作用产生的超氧化物歧化酶，促进蛋白质分子的半胱氨酸残基间形成二硫键。紫杉醇被用作为模型药物制备了靶向FR的微粒FA-BSA-PTX。对微粒进行了的粒径，包封率进行了检测，对其物理化学性质进行了表征。对不同交联剂二次固化获得的微粒的体外释放特性进行比较。FA-BSA-PTX对于Hela细胞的摄取情况被通过激光共聚焦显微镜(LCSM)评估了。根据获得的数据，具有靶向性能的BSA-PTX纳米粒子在本研究中被通过利用该新颖的绿色化学方法成功地制备了。结果与讨论凝固剂的筛选根据文献报道，分子间功能区可通过蛋白质分子和二价金属离子之间相互作用产生，形成沉淀物，这可用于增强蛋白质作用的稳定性和持续时间，以达到缓释效果。而且，凝固剂种类可能对白蛋白的交联效果以及交联速率都会产生影响，进而会影响到白蛋白微粒的粒径和白蛋白转化率。所以，在本研究中，以粒径和转化率为指标，不同的凝固剂对这两项指标的影响被考察了。如图2所示，在相同的制备条件下，加入50 mg凝固剂组（1.25mg/mL）的转化率比添加20 mg凝固剂组（0.5 mg/mL）的高。对于0.5 mg/mL凝固剂组转化率的次序：氯化镁>葡萄糖酸内酯>氯化钙>乳酸钙，其中以氯化镁作为凝固剂的转化率最高（47%）。但是在使用葡萄糖酸内酯作为凝固剂时，微粒会产生明显的絮凝。颗粒之间无法有效的分散。同样，0.5 mg/mL凝固剂组制备的白蛋白微粒的粒径要比1.25 mg/mL凝固剂组的粒径小，对于0.5 mg/mL凝固剂组粒径大小的次序：氯化镁

0/5000

源语言: -

目标语言: -

结果 (英语) 1: [复制]

复制成功！

However, the morphologically formed nanoparticles are not stable and need to be hardened by cross-linking with glutaraldehyde. However, adverse effects such as the inactivation of biologically active macromolecules and the residue of harmful solvents will be caused. In short, acetone, chloroform, dichloromethane and other medium and highly toxic organic solvents need to be added in the preparation process by the above methods, and certain pollution will be caused accordingly. Green chemistry is a powerful ally in the pharmaceutical industry. From the perspective of green chemistry, two main steps must be completed in the process of preparing nanoparticles: choosing the solvent medium for synthesis and choosing non-toxic materials for stabilizing the nanoparticles. Most of the preparation methods reported so far rely heavily on organic solvents. In contrast, the development of environmentally friendly and sustainable methods needs to be proposed to prepare nanoparticles in the green synthesis strategy. In this research, a new green method for preparing albumin particles (high pressure homogenization-coagulation method) is proposed. In the water system, albumin nanoparticles are formed by using the action of a coagulant to coagulate the albumin molecules, and at the same time using the superoxide dismutase produced by the high-pressure homogeneous cavitation to promote the cysteamine of the protein molecules Disulfide bonds are formed between acid residues. Paclitaxel was used as a model drug to prepare FR-targeted microparticles FA-BSA-PTX. The particle size and encapsulation efficiency of the particles were tested, and their physical and chemical properties were characterized. The in vitro release characteristics of microparticles obtained by secondary curing of different crosslinking agents were compared. The uptake of FA-BSA-PTX to Hela cells was evaluated by confocal laser microscopy (LCSM). According to the data obtained, BSA-PTX nanoparticles with targeting properties were successfully prepared in this study by using this novel green chemical method. Results and discussion Screening of coagulants According to reports in the literature, intermolecular functional regions can be produced by the interaction between protein molecules and divalent metal ions to form precipitates, which can be used to enhance the stability and duration of protein action to achieve relaxation Interpretation effect. Moreover, the type of coagulant may affect the cross-linking effect and cross-linking rate of albumin, which in turn affects the particle size of albumin particles and the albumin conversion rate. Therefore, in this study, the particle size and conversion rate were used as indicators, and the influence of different coagulants on these two indicators was investigated. As shown in Figure 2, under the same preparation conditions, the conversion rate of the 50 mg coagulant group (1.25 mg/mL) was higher than that of the 20 mg coagulant group (0.5 mg/mL). For the 0.5 mg/mL coagulant group, the order of conversion rate is: magnesium chloride>gluconolactone>calcium chloride>calcium lactate. Magnesium chloride has the highest conversion rate (47%). However, when gluconolactone is used as a coagulant, the particles will produce obvious flocculation. The particles cannot be effectively dispersed. Similarly, the particle size of albumin particles prepared in the 0.5 mg/mL coagulant group is smaller than that of the 1.25 mg/mL coagulant group. For the 0.5 mg/mL coagulant group, the order of particle size: magnesium chloride

正在翻译中..

结果 (英语) 2:[复制]

复制成功！

However, the nanoparticles that have formed in morphology are not stable and need to be hardened by crosslinking such as dialdehyde. However, adverse effects, such as bioactive polymer ineration and residues of harmful solvents, can be caused. In short, highly toxic organic solvents such as acetone, chloroform and dichloromethane need to be added to the preparation process by the above methods, and certain contamination will be caused accordingly. Green Chemistry is a strong ally of the pharmaceutical industry. From a green chemistry perspective, two main steps must be taken in the preparation of nanoparticles: the selection of solvent media for synthesis and the selection of non-toxic materials for stabilizing nanoparticles. Most of the preparation methods reported so far rely heavily on organic solvents. In contrast, the development of environmentally friendly and sustainable methods needs to be proposed to prepare nanoparticles in green synthesis strategies. In this study, a new method of preparing albumin particles in green (high-pressure homogeneity-coagulation) is proposed. In the water system, albumin nanoparticles are formed by using the act of coagulants to solidify albumin molecules, and by using the hollowing out of high-pressure homogeneity to produce superoxide dismigases to promote the formation of disulfur bonds between the cysteine residues of protein molecules. Yew alcohol is used as a model drug to prepare THE-targeted particles FA-BSA-PTX. The particle size and envelope rate were detected and its physical and chemical properties were characterted. The in-body release characteristics of particles obtained from secondary curing of different crosslinkers were compared. FA-BSA-PTX's intake of Hela cells was evaluated by a laser confocus microscope (LCSM). According to the data obtained, BSA-PTX nanoparticles with targeted properties were successfully prepared in this study using this novel green chemical method. Results and discussions. Screening of coagulants. According to literature, the intermodal functional area can be produced by interaction between protein molecules and priceless metal ions to form sediments, which can be used to enhance the stability and duration of protein action in order to achieve a slow release effect. In addition, the type of curing agent may have an effect on the crosslinking effect of albumin and the interlinking rate, which in turn may affect the particle size and albumin conversion rate of albumin particles. Therefore, in this study, the effects of different coagulants on these two indicators were examined by particle size and conversion rate. As shown in Figure 2, under the same preparation conditions, the conversion rate of the 50 mg coagulant group (1.25 mg/mL) is higher than that of the 20 mg coagulant group (0.5 mg/mL). For the 0.5 mg/mL coagulant group conversion rate order: magnesium chloride >intragluctrate >calchloride chloride> calcium lactate, of which magnesium chloride as a coagulant conversion rate was the highest (47%). However, when glucate esters are used as coagulants, particles produce significant flocculation. There is no effective dispersion between particles. Similarly, the particle size of albumin particles prepared in the 0.5 mg/mL coagulant group is smaller than that of the 1.25 mg/mL coagulant group, and the order of particle size of the 0.5 mg/mL coagulant group is magnesium chloride.

正在翻译中..

结果 (英语) 3:[复制]

复制成功！

However, the nanoparticles formed in morphology are not stable and need to be hardened by crosslinking with glutaraldehyde. However, adverse effects such as deactivation of bioactive macromolecules and residues of harmful solvents can be caused. In a word, acetone, chloroform and dichloromethane should be added in the preparation process according to the above method, which will cause pollution. Green chemistry is a strong ally of the pharmaceutical industry. From the perspective of green chemistry, two main steps need to be completed in the preparation of nanoparticles: the selection of solvent medium for synthesis and the selection of non-toxic materials for stabilizing nanoparticles. Most of the preparation methods reported so far rely heavily on organic solvents. In contrast, the development of environmentally friendly and sustainable methods need to be proposed to prepare nanoparticles in green synthesis strategies. In this study, a new green method (high pressure homogenization coagulation method) for the preparation of albumin particles was proposed. In the water system, albumin nanoparticles are formed by coagulant, and superoxide dismutase (SOD) produced by high pressure homogenization can promote the formation of disulfide bond between cysteine residues of protein molecules. Paclitaxel was used as a model drug to prepare fa-bsa-ptx particles targeting fr. The particle size, entrapment efficiency and physicochemical properties of the particles were characterized. The in vitro release characteristics of the particles cured by different crosslinking agents were compared. The uptake of fa-bsa-ptx in HeLa cells was evaluated by laser confocal microscopy (LCSM). According to the data obtained, bsa-ptx nanoparticles with targeted properties were successfully prepared by using this novel green chemical method. Results and discussion Selection of coagulant According to the literature reports, the intermolecular functional regions can be generated by the interaction between protein molecules and divalent metal ions to form precipitates, which can be used to enhance the stability and duration of protein interaction to achieve sustained release effect. Moreover, coagulant type may affect the crosslinking effect and crosslinking rate of albumin, and then affect the particle size and conversion of albumin. Therefore, in this study, the effects of different coagulants on the particle size and conversion were investigated. As shown in Figure 2, under the same preparation conditions, the conversion rate of 50 mg coagulant group (1.25 mg / ml) was higher than that of 20 mg coagulant group (0.5 mg / ml). For 0.5 mg / ml coagulant group, the order of conversion rate was magnesium chloride > gluconolactone > calcium chloride > calcium lactate, and magnesium chloride as coagulant had the highest conversion rate (47%). However, when gluconolactone is used as coagulant, the particles will produce obvious flocculation. There is no effective dispersion between particles. Similarly, the particle size of albumin particles prepared in 0.5 mg / ml coagulant group was smaller than that in 1.25 mg / ml coagulant group. For 0.5 mg / ml coagulant group, the order of particle size was magnesium chloride

正在翻译中..

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