Overexpress and knock down ANTXR1 in k562 cell line, cord blood CD34+ cells, and HUDEP-2 cell line to check the changes to γ-globin. The effect of Wnt/β-catenin pathway on γ-globin was analyzed by RT-qpcr, western blot analysis, immunofluorescence technology, TOP/FOP experiment, nucleoplasmic separation experiment, CHIP-QPCR. At the same time, K562 cells were treated with different concentrations of Wnt/β-catenin inhibitor XAV939 and activator LICI to detect the effect of ANTXR1 in the Wnt/β-catenin pathway on γ-globin.
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