β-半乳糖苷酶检测试剂盒染色和流式细胞术检测氯马斯汀对肿瘤细胞的影响。

β-galactosidase detection kit staining and flow cytometry were used to detect the effect of clemastine on tumor cells. The statistical results show that clomastine treatment of U87 and U251 cells significantly increased the proportion of senescent cells (P <0.05). Western Blot detection found that U87 and U251 cells treated with clomastine showed that Skp2 levels decreased significantly and p21 / p27 levels increased significantly (P <0.05).

Beta-semilactose detection kit staining and flow cytometry detect the effects of chlormastin on tumor cells. Statistical results showed that the treatment of U87 and U251 cells by chlormastin resulted in a significant increase in the proportion of senescent cells (P 0.05). Western Blot detected U87 and U251 cells treated with chlormastin and found a significant decrease in Skp2 levels, with a significant increase in p21/p27 levels (P 0.05).

β - galactosidase detection kit staining and flow cytometry were used to detect the effect of lomastatin on tumor cells. The statistical results showed that the percentage of senescent cells increased significantly (P < 0.05) zero point zero five ）。 Western blot showed that the Skp2 level decreased and p21 / p27 level increased significantly (P < 0.05) zero point zero five ）。<br>