Thirty samples were taken and divided into three groups, with ten samples in each group. A total of 3 bottles of solutions were prepared by injecting half of each sample in the first group and the second group into 100ml of pH7.0 sodium chloride-peptone buffer, and the remaining half of the first group and the second Half of each sample in the three groups was injected into 100ml of pH7.0 sodium chloride-peptone buffer, and the remaining half of the second group and the remaining half of the third group were injected into 100ml of pH7.0 sodium chloride-peptone buffer The solution was then rotated left and right to mix evenly and used as the test solution.
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