Later, in cells overexpressing E2F2, the expression of deoxycytidine kinase DCK, a gene related to gemcitabine resistance in RRM2 and its downstream, was up-regulated. Knockdown of E2F2 was verified on the contrary.
Later, in cells that had expressed E2F2, RRM2 and its downstream expression of DCK, a gene associated with giesythabin resistance, was increased, and the results of knocking down E2F2 were verified instead.