所构建的两种免疫脂质磁珠被用于对入组的肺癌患者进行肿瘤细胞的捕获，以统

所构建的两种免疫脂质磁珠被用于对入组的肺癌患者进行肿瘤细胞的捕获，以统计CTC的数量，结果如图3A所示。在入组的35例患者中，两种IML均可以分离到肺癌CTC。然而，统计发现与传统的EpCAM免疫脂质磁珠相比，EGFR脂质磁珠能够分选得到更多的肺癌CTC，对肺癌CTC具有更好的特异性和捕获效率。此外，两种磁球对患者血液中CTC的分选结果如图3B所示，检测到的CTC在经过免疫荧光染色，拍照结果表明对两种体液分选得到的CTC形态大小接近，染色状态也非常一致，构建的两种免疫脂质磁球可以有效分离肺癌患者外周血中的CTC。

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结果 (英语) 1: [复制]

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The constructed two kinds of immunolipid magnetic beads were used to capture tumor cells of the enrolled lung cancer patients to count the number of CTCs. The results are shown in Figure 3A. Among the 35 patients enrolled, both types of IML can be isolated from lung cancer CTC. However, statistics found that compared with traditional EpCAM immunolipid magnetic beads, EGFR lipid magnetic beads can sort more lung cancer CTCs, and have better specificity and capture efficiency for lung cancer CTCs. In addition, the sorting results of the two kinds of magnetic balls for the CTC in the patient's blood are shown in Figure 3B. The detected CTCs were stained by immunofluorescence. The photographed results showed that the CTCs sorted from the two body fluids were similar in shape and size, and the staining state was also Very consistent, the constructed two kinds of immunolipid magnetic spheres can effectively separate CTC in peripheral blood of lung cancer patients.

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结果 (英语) 2:[复制]

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The two immunolipid magnetic beads were constructed to capture tumor cells in lung cancer patients in the group to count the number of CTC, as shown in Figure 3A. In the 35 patients in the group, both IMLs were isolated to the lung cancer CTC. However, it was found that compared with the traditional EpCAM immunolipid beads, EGFR lipid beads were able to pick and choose more lung cancer CTC, which had better specificity and capture efficiency for lung cancer CTC. In addition, the two magnetic balls in the patient's blood CTC selection results shown in Figure 3B, detected CTC in the immunofluorescence staining, the photo results show that the two body fluids are selected to obtain the CTC form size is close, dyeing status is also very consistent, the construction of two immunolipid magnetosomes can effectively isolate lung cancer patients in the outer blood CTC.

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结果 (英语) 3:[复制]

复制成功！

The constructed two kinds of immunolipid magnetic beads were used to capture tumor cells of lung cancer patients in order to count the number of CTC. The results are shown in Figure 3a. In 35 patients, both IML and IML could be separated into lung cancer CTC. However, compared with the traditional EpCAM immunomagnetic beads, EGFR immunomagnetic beads can obtain more lung cancer CTC, and have better specificity and capture efficiency for lung cancer CTC. In addition, the sorting results of two kinds of magnetic spheres for CTC in patients' blood are shown in Fig. 3B. The detected CTC is stained by immunofluorescence. The photographing results show that the shape and size of CTC obtained by sorting two kinds of body fluids are similar, and the staining state is also very consistent. The two kinds of immunolipid magnetic spheres can effectively separate CTC in peripheral blood of patients with lung cancer.

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