钙依赖蛋白激酶（GhCPK4）对棉花黄萎病的调控机制研究摘要：黄萎病是

Research on the regulation mechanism of calcium-dependent protein kinase (GhCPK4) on cotton verticillium wilt <br>Abstract: Verticillium wilt is a cancer of cotton, which seriously affects cotton yield and quality. major scientific issues. CPK (calcium-dependent protein kinase) is a calcium ion receptor in higher plants, which is involved in the regulation of plant responses to various environmental stresses. Based on the previous proof that GhCPK4 gene is involved in the negative regulation of cotton verticillium wilt, this project intends to study its sequence and gene structure, and analyze its biological information; use yeast two-hybrid to find its interacting protein, and study GhCPK4 in vitro and in vivo experiments To explore the molecular basis of GhCPK4 gene's involvement in the signal regulation of cotton verticillium wilt resistance; using gene editing technology CRISPR/Cas9 to mutate and overexpress GhCPK4 gene site-specifically, to clarify its involvement in cotton resistance to verticillium wilt Molecular mechanisms of defense reactions. In this way, the molecular regulation mechanism of GhCPK4 gene in the immune regulation of cotton resistance to verticillium wilt can be explored, which can provide solutions and new disease resistance genes for genetic engineering methods to improve cotton verticillium wilt resistance, and promote the development of cotton disease resistance molecular breeding. Lay the foundation for genetic improvement of cotton and the breeding of new varieties. <br>Key words: cotton; verticillium wilt; GhCPK4 gene; molecular breeding

Regulation mechanism of calcium dependent protein kinase (ghcpk4) on Cotton Verticillium Wilt<br>The genetic mechanism of Cotton Verticillium wilt resistance and quality improvement has been a major problem in the field of cotton breeding. CPK (calcium dependent protein kinase) is a calcium receptor in higher plants, which is involved in regulating the response of plants to a variety of environmental stresses. Based on the previous proof that ghcpk4 gene is involved in the negative regulation of Cotton Verticillium wilt, this project intends to study its sequence and gene structure and analyze its biological information; Yeast diza was used to find its interaction protein, and the interaction relationship between ghcpk4 interaction proteins was studied in vivo and in vitro, so as to explore the molecular basis of ghcpk4 gene involved in the signal regulation of cotton resistance to Verticillium wilt; Gene editing technology CRISPR / cas9 was used to site mutation and overexpression of ghcpk4 gene, so as to clarify its molecular mechanism involved in the defense response of cotton to Verticillium wilt. So as to explore the molecular regulation mechanism of ghcpk4 gene in the immune regulation of Cotton Verticillium wilt resistance, provide solutions and new disease resistance genes for genetic engineering to improve cotton Verticillium wilt resistance, promote the development of cotton disease resistance molecular breeding, and lay a foundation for cotton genetic improvement and new variety cultivation.<br>Key words: cotton; Verticillium wilt; Ghcpk4 gene; molecular breeding

Mechanism of calcium-dependent protein kinase (GhCPK4) regulating cotton verticillium wilt Verticillium wilt is a cancer of cotton, which seriously affects the yield and quality of cotton. Genetic improvement and disease resistance mechanism of verticillium wilt have been a major scientific issue in the field of cotton genetics and breeding. Cpk (calcium-dependent protein kinase) is a calcium receptor in higher plants, which is involved in regulating the response of plants to various environmental stresses. This project intends to study its sequence and gene structure and analyze its biological information on the basis of proving that GhCPK4 gene participates in the negative regulation of cotton verticillium wilt. The yeast hybrid was used to find its interacting protein, and the interaction between GhCPK4 interacting proteins was studied in vitro and in vivo to explore the molecular basis of GhCPK4 gene's participation in the signal regulation of cotton resistance to verticillium wilt. Gene editing technology CRISPR/Cas9 was used to make site-directed mutation and overexpression of GhCPK4 gene, and the molecular mechanism of its participation in cotton defense against verticillium wilt was clarified. Therefore, we can find out the molecular regulation mechanism of GhCPK4 gene in the immune regulation of cotton resistance to verticillium wilt, provide solutions and new resistance genes for improving cotton resistance to verticillium wilt by means of genetic engineering, promote the development of molecular breeding of cotton disease resistance, and lay a foundation for cotton genetic improvement and new variety cultivation. Key words: cotton; Verticillium wilt; GhCPK4 gene; Molecular breeding