目前，许多与非生物胁迫相关的MYB基因在拟南芥中已相继得到克隆与功能鉴

At present, many MYB genes related to abiotic stress have been cloned and functionally identified in Arabidopsis, but the corresponding studies in woody plants such as apples and fruit trees are still very few. Their metabolic digestion genomes involved in abiotic stress After DNA, the RNA was reverse transcribed into cDNA, and the 18S conservative specific primer was used to perform PCR detection on the cDNA template. The target band of about 100 bp was amplified, indicating that the reverse transcription was successful and the cDNA template can be used in subsequent experiments. The mechanism of regulation is unclear.

At present, many myB genes related to non-biological stress have been cloned and functionally identified in amoeba, but there are few corresponding studies in woody plant apples and other fruit trees, and after participating in the metabolic digestion of genomic DNA of abiotic stress, RNA is transcribed into cDNA, pcR testing of cDNA templates with 18S conservative specific primers, and amplifying the expected size band of about 100 bp, indicating that the reverse transcription can be used for subsequent testing. The regulatory mechanism is not clear.

At present, many MYB genes related to abiotic stress have been cloned and identified successively in Arabidopsis, but there are few studies on them in woody plant apple and other fruit trees. After the metabolizing and digesting genomic DNA involved in abiotic stress, the RNA is retrotranscribed into cDNA, and the cDNA template is detected by PCR with 18S conservative specific primers, and about 100 genes are amplified The expected size of the target band of BP indicates that the reverse transcription is successful, and the cDNA template can be used for subsequent experiments. The mechanism of regulation is not clear.<br>