确认方法：取100ml灭菌前的样品，经直径为50mm，孔径为0.45μ

Confirmation method: Take 100ml of the sample before sterilization and filter it through a filter membrane with a diameter of 50mm and a pore size of 0.45μm. Take out the filter membrane, put it in a sterile test tube and place it in a boiling water bath, keep it for 15 minutes, take it out, and cool it quickly. Add 50 ml of thioglycolate fluid medium. Use sterile normal saline to operate as above to prepare a negative control. Cultivation: Place the test tube in a water-proof constant temperature incubator at 30-35°C for 7 days and observe the results daily. <br>Acceptable criteria: should grow aseptically.

Confirmation method: Take 100 ml of pre-sterilization samples, filtered by a membrane with a diameter of 50mm and a aperture of 0.45 m. Remove the membrane, put it in a sterile test tube and place in a boiling water bath, hold it for 15 minutes, remove it and cool quickly. Add 50 ml of sulfur ethanolate fluid media. Using sterile physiological saline water according to the above method, preparation of negative control. Culture: The test tube was cultured for 7 days in a water-ion thermostat culture box for 7 days and observed day by day.<br>Acceptable standard: sterile growth.

Confirmation method: take 100ml sample before sterilization and filter it through a filter membrane with diameter of 50mm and pore diameter of 0.45 μ M. Take out the filter membrane, put it into a sterile test tube, put it in a boiling water bath, keep it for 15 minutes, take it out and cool it quickly. 50 ml thioglycollate fluid medium was added. Sterile normal saline was used to prepare negative control. Culture: put the test tube in the water-proof constant temperature incubator for 7 days at 30-35 ℃, and observe the results day by day.<br>Acceptable criteria: aseptic growth.