用胰蛋白酶将细胞制备成单细胞悬液，并加入96孔板中培养，每孔细胞为80

Use trypsin to prepare the cells into a single cell suspension and add them to a 96-well plate for culture. There are 8000 cells per well. When the cells grow to 80%, replace them with DMEM containing different amounts of Ep-LMB and Vi-LMB. 100μL of complete culture medium, blank control plus 100μL of DMEM complete medium to continue culturing.

The cells were prepared with trypsin into a single cell suspension and cultured in a 96-well plate, with 8000 cells per hole, and when the cells grew to 80%, they were replaced with a full culture of 100 sl containing different amounts of Ep-LMB, Vi-LMB DMM, and the blank control plus DMMEM full culture fluid 100 sL continued culture.

The cells were prepared into single cell suspension with trypsin and cultured in 96 well plate. The number of cells in each well was 8000. When the cells grew to 80%, they were replaced with 100 μ l DMEM complete culture medium containing different amounts of EP LMB and VI LMB, and the blank control was added with 100 μ l DMEM complete culture medium.<br>