从红外光谱中看到酰胺Ⅰ键的吸收带位置有移动，证明蛋白质的构象中的改变。

The shift of the absorption band position of the amide I bond can be seen from the infrared spectrum, which proves the change in the protein conformation. <br>The Raman spectrum shows that the disulfide bond configuration has changed and the protein structure has also changed. <br>In vitro release studies have confirmed its sustained release properties, which can achieve 6 hours of sustained release after cross-linking with a cross-linking agent, and the release rate using different cross-linking agents is genipin>glutaraldehyde>vanillin. <br>In summary, the high-pressure homogenization-coagulation method for preparing albumin drug-loaded particles proposed in this paper is a simple and feasible method with great potential. <br>On the other hand, only bovine serum albumin was used in this study, and only four coagulants and three cross-linking agents were evaluated. Other types of albumin, coagulants and cross-linking agents need to be further studied in the future. .

The position of the absorption band of the alamide I. key is seen in the infrared spectrum, which proves the change in the composition of the protein.<br>Raman spectra show that the configuration of the desulfur bond has changed, and so has the protein structure.<br>In vitro release studies have confirmed its slow release properties, which can be continuously released for up to 6 hours after crosslinking with crosslinking agents, and the release rate using different crosslinkers is gynipin >dealdehyde> vanilla aldehyde.<br>In summary, the high-pressure homogeneity-coagulation method proposed in this paper for the preparation of albumin-carrying particles is a simple and feasible method with great potential.<br>On the other hand, only bovine serum albumin was used in this study, and the effects of only 4 coagulants and 3 crosslinkers were evaluated, and the effects of other types of albumin, coagulants and crosslinkers have yet to be further studied in the future.

The infrared spectrum showed that the position of the absorption band of amide I bond shifted, which proved the change of protein conformation.<br>The Raman spectra showed that the disulfide bond configuration changed and the protein structure changed accordingly.<br>The in vitro release study confirmed the sustained release characteristics of the drug. The sustained release rate of genipin > glutaraldehyde > vanillin was achieved after crosslinking with crosslinking agent.<br>In conclusion, the high pressure homogenization coagulation method proposed in this paper is a simple and feasible method with great potential.<br>On the other hand, only bovine serum albumin (BSA) was used in this study, and the effects of four coagulants and three crosslinking agents were evaluated. The other kinds of albumin, coagulants and crosslinkers need to be further studied in the future.<br>